Journal: Frontiers in Immunology

Article Title: Immune escape pathways from the HBV core 18-27 CD8 T cell response are driven by individual HLA class I alleles

doi: 10.3389/fimmu.2022.1045498

Figure Lengend Snippet: The core 18-27 epitope is restricted by multiple HLA class I molecules. PBMCs from 154 patients with chronic HBV infection were stimulated for 10 days in the presence of the core 18-27 peptide and re-stimulated for 5h before IFNγ production was determined by ICS. Depicted are IFNγ producing HBV core 18-27 specific CD8 T cells, the IFNγ response from non-stimulated cell was subtracted (A) IFNγ responses according to the HBeAg status. (B) IFNγ responses according to the HLA-type of the HBeAg negative patients from (A) . Patients depicted with single HLA-types had no other relevant HLA-type (e.g. A*02:01 are patients negative for HLA-B*35:01/03 or HLA-B*51 and vice versa), patients with “multiple alleles” had at least two relevant HLA-alleles (e.g. A*02:01 and B*51). (C) The HLA-restriction of antigen-specific CD8 + T cells was determined with partially HLA-matched peptide-pulsed target cells expressing only HLA-A*02:01 (not B*35 or B*51), B35:01/03 (not A*02:01 or B*51), HLA-B*51 (not A*02:01 or B35), others (not A*02:01, B*35 or B*51). As control core18-27 peptide was directly added to the culture (peptide). The frequency of IFNγ -producing CD8 + T cells was determined by ICS and a representative example is shown in Supplemental Figure S3 .

Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation (Biocoll; Biochrom) ( ).

Techniques: Infection, Expressing, Control